PLAMseq is a new technique that enables simultaneous analysis of chromatin-associated proteins and their genomic locations in a single experiment, using TurboID to biotinylate nearby proteins and capture both proteins and DNA fragments.

Further information can be found in the Science Advances paper by Lourdes González-Vinceiro et al., with DOI 10.1126/sciadv.ady4151.

The method could accelerate discovery of therapeutic targets and biomarkers by advancing understanding of epigenetic mechanisms in diseases such as cancer and neurodevelopmental disorders.

The study positions Andalusian research groups as leaders in epigenetics and chromatin biology technologies, attracting growing international interest to apply PLAMseq across disciplines.

Developed in Seville at CABIMER and IBiS, the technology marks a significant advance in proteogenomics and chromatin biology.

Demonstrations with core genome proteins such as CTCF, RNA polymerase II, and SUMO-modified H1 histones uncovered novel interactions, including SETDB1 binding to modified histones.

PLAMseq overcomes prior limits that demanded multiple complex, costly techniques like ChIP-seq and mass spectrometry and relied on highly specific antibodies.

Román González-Prieto notes that PLAMseq reveals both the proteins shaping the genome and the genomic regions they act upon in a single experiment, offering a dual-dimensional view of genome biology.