Leading contributions came from authors affiliated with the University of Fribourg and the University of British Columbia, who drove concepts, experiments, data analysis, visualization, and manuscript writing, with C.R.-R., P.C.B., G.S., E.C., D.Á., M.D., and S.V. taking leading roles.

The study situates its TMEM-170 work within a broad methodological context that references lipid scramblase roles in autophagy and membrane biogenesis, and highlights bridge-like lipid transfer proteins as part of the ER lipid trafficking network.

The paper includes extensive methodological references and data-supporting materials, with datasets and source data linked to the Nature Structural & Molecular Biology publication (2025) and its Sec14 section to enable validation and reuse.

Data availability is detailed: the cryo-EM map of the C. elegans LPD-3 TMEM-170 Spigot complex (EMD-45399), its high-resolution structure (PDB 9CAP), as well as MD trajectories and analysis scripts, are accessible on Zenodo.

Acknowledgements credit funding from the Swiss National Science Foundation, the European Research Council, the Canadian Institutes of Health Research, and BC Children’s Hospital Research Institute, supporting both computational and experimental aspects.