The ceremony honoring O'Brien will be available for streaming on Netflix starting May 4, 2025, showcasing the legacy of past recipients like George Carlin and Whoopi Goldberg.

David Letterman presented the award to O'Brien, who expressed surprise and gratitude to receive it from such a legendary figure.

Trump's board is now predominantly Republican, leading to resignations from various consultants and artists opposed to his administration's policies, which has impacted the center's artistic direction.

Comedian Sarah Silverman humorously referenced Trump's controversial remarks about women, aligning them with the event's overall critique of his leadership.

The ceremony raised questions about the future of political humor in light of Trump's influence over the Kennedy Center's administration, which has shifted focus towards political discourse since his takeover.

On February 24, 2025, leading comedians gathered at the Kennedy Center to honor Conan O'Brien with the Mark Twain Prize for American Humor, using the occasion to critique Donald Trump's recent takeover of the center.

During his acceptance speech, O'Brien expressed gratitude to former president Deborah Rutter and board chairman David Rubenstein, while highlighting the uncertainty surrounding the Kennedy Center's future.

The Kennedy Center, once a space for bipartisan recognition of artistic achievement, is now facing the possibility of reflecting Trump's partisan tastes, which some critics fear could undermine its original mission.

In his remarks, O'Brien drew parallels between Mark Twain's disdain for bullies and current events, emphasizing the importance of empathy for the weak.

Notably absent from the ceremony, Trump has continued a pattern of non-participation during his presidency, underscoring the tension between the institution and his administration.

O'Brien emphasized the importance of honoring Mark Twain and supporting the Kennedy Center staff during this turbulent period, reinforcing the values represented by the award.

Reflecting on his career, O'Brien expressed disbelief at being honored 40 years later at such a prestigious venue, underscoring his journey in comedy.